25-Hydroxy Vitamin D Test

Also Known As:  Ergocalciferol (Vitamin D₂), Cholecalciferol (Vitamin D₃). Calcidiol (25-hydroxyvitamin D), Calcifidiol (25-hydroxy-vitamin D), Calcitriol (1,25 dihydroxy-vitamin D), 25-hydroxyvitamin D, 1,25 dihydroxyvitamin D, Vitamin D

Test panel: Vitamin D, Vitamin A, Vitamin B, Vitamin B6, Vitamin B₁₂, Vitamin C, Vitamin E, Vitamin K, Calcium

Why Det Tested:

• Indicated in the postmenopausal women to evaluate Vit.D level which is needed for the absorption of Calcium.
• To evaluate the role of Vit.D in osteoporosis.
• To find the role of Vit.D in the prevention of cancer.

When To Get Tested:

• When you have an abnormal calcium.
• When you have an abnormal phosphorus.
• When you have an abnormal parathyroid hormone level.
• When you have evidence of bone disease or bone weakness.
• When healthcare practitioner suspects that you might have a vitamin D deficiency.

Required Sample

1. Venous blood is needed to prepare the serum.
2. The random sample can be used.
3. The sample is stable at room temperature for 72 hours.
   1. The sample is stable at 22 to 25 °C.

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People at risk of Vit.D deficiency are:

• Infants on breastfeeding (human milk is deficient in Vit.D).
• Occupation when people are not exposed to the sun.
• Individual too fatty with body mass index (BMI) >30 because Vit.D is trapped in fatty tissues.
• Malabsorption from GIT.
• Patient with liver or kidneys diseases.

Normal Values:

• Indicate vit.D deficiency = <20 ng/mL 
•  Insufficiency of vit.D = 20 to 30 ng/mL
•  Optimal level of vit.D = >30 ng/mL
• Toxic level of vit.D = >200 ng/mL
  • Recommended dose of vitamin D for adult is 5 µg/day.

Increased level of Vit.D in Seen in:

• Excessive dietary supplement.
• William syndrome. This is a rare genetic disorder.

Decreased level of Vit.D is Seen in :

• Rickets.
• Osteoporosis.
• Liver diseases.
• Renal diseases.
• Inadequate dietary intake.
• Inadequate exposure to sunlight.
• Acute inflammatory diseases.
• Familial hypophosphatemic rickets.

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