For the Measurement of the Resistance and Sensitivity of the major microbial species to the antibiotic/chemothraputic agents.
PRINCIPLE:
The discs-diffusion test is based on the fact that for a given antibiotic, the size of the zone inhibition is inversely related to MIC (determined by dilution method) of the strain being tested when the test conditions are held constant. Antimicrobial susceptibility testing with discs is a simple and rapid method and provides a reproducible means of testing bacterial sensitivity to various antibiotics and chemotherapeutic agents.
List of Antibiotic Discs:
| Antimicrobial Agent | Test organisms |
|---|---|
| Amikacin (AK) | Entrobacteriaceae Pseudomonas aeruginosa Acinotobector spp Staphylococcus spp |
| Amoxycillin (AX) | All organisms |
| Amoxycillin/ Clavulanic acid (AC) | Entrobacteriaceae Staphylococcus spp. Heamophilius spp. H.parainfluenzae. |
| Ampicillin (AP) | Entrobacteriaceae Vibro cholera Staphylococcus spp. Enterococcus spp. Heamophilius spp. H.parainfluenzea Streptococcus spp. |
| Ampicillin/ Sulbactam (AS) | Entrobacteriaceae Acinetobacter spp. Staphylococcus spp. Heamophilius spp. H.parainfluenzea |
| Azithromycin (AZ) | Staphylococcus spp. Streptococcus spp. Heamophilius spp. H.parainfluenzea Neisseria meningitides |
| Azterionam (AT) | Entrobacteriaceae Pseudomonas aeruginosa. Acinetobacter spp. Heamophilius spp. H.parainfluenzea |
| Cefaclor (CG) | Entrobacteriaceae Staphylococcus spp. Heamophilius spp. H.parainfluenzea |
| Cefadroxile (CD) | All organisms |
| Cefazolin (CF) | Entrobacteriaceae Staphylococcus spp. |
| Cefdinir (CN) | Entrobacteriaceae Staphylococcus spp. Heamophilius spp. H.parainfluenzea |
| Cefixime (FX) | Entrobacteriaceae Heamophilius spp. Neisseria meningitides |
| Cefoperazone (PZ) | Entrobacteriaceae Pseudomonas aeruginosa. Staphylococcus spp. |
| Cefoperazone /sulbactum (CS) | Pseudomonas aeruginosa. Acinetobacter spp. Staphylococcus spp. |
| Cefotaxime (CX) | Entrobacteriaceae Pseudomonas aeruginosa. Acinetobacter spp. Staphylococcus spp. Heamophilius spp. H.parainfluenzea Neisseria gonorrhoeae. Staphylococcus spp. Neisseria meningitides |
| Cefpirome (CE) | Entrobacteriaceae Acinetobacter spp. Pseudomonas aeruginosa. |
| Cefpodoxime (CO) | Entrobacteriaceae Staphylococcus spp. Heamophilius spp. H.parainfluenzea |
| Cefprozil (FP) | Entrobacteriaceae Staphylococcus spp. Heamophilius spp. H.parainfluenzea |
| Ceftazidime (CZ) | Entrobacteriaceae Pseudomonas aeruginosa. Acinetobacter spp. Staphylococcus spp. Heamophilius spp. H.parainfluenzea Burkholderia cepacia Neisseria gonorrhoeae. |
| Ceftizoxime (FO) | Entrobacteriaceae Pseudomonas aeruginosa. Acinetobacter spp. Staphylococcus spp. Heamophilius spp. H.parainfluenzea Neisseria gonorrhoeae. |
| Ceftriaxone (FR) | Entrobacteriaceae Pseudomonas aeruginosa. Acinetobacter spp. Staphylococcus spp. Heamophilius spp. H.parainfluenzea Neisseria gonorrhoeae. Streptococcus spp Neisseria meningitides |
| Cefuroxime Sodium (CR) | Entrobacteriaceae Staphylococcus spp. Heamophilius spp. H.parainfluenzea Neisseria gonorrhoeae. |
| Cephalexin (CP) | All Organisms |
| Cephaloridine (CH) | All Organisms |
| Cephalothin (CA) | Entrobacteriaceae Staphylococcus spp. |
| Chloramphenicol (CK) | Entrobacteriaceae Staphylococcus spp. Enterococcus spp. Vibrio cholerea Heamophilius spp. H.parainfluenzea Neisseria meningitides Streptococcus pneumoniae Streptococcus spp |
| Ciprofloxacin (CI) | Entrobacteriaceae Pseudomonas aeruginosa. Acinetobacter spp. Staphylococcus spp. Enterococcus spp. Heamophilius spp. H.parainfluenzea Neisseria gonorrhoeae Neisseria meningitides |
| Clarithromycin (CL) | Staphylococcus spp. Heamophilius spp. H.parainfluenzea Streptococcus spp. |
| Clindamycin (CM) | Staphylococcus spp. Streptococcus spp. |
| Claxacillin (CV) | All organisms |
| Co-Trimoxazole (CT) | Entrobacteriaceae Acinetobacter spp. Staphylococcus spp. Heamophilius spp. H.parainfluenzea Vibrio cholerea Burkholderia cepacia Stenotrophomonas maltophilia Streptococcus pneumoniae Neisseria meningitides |
| Doxycycline (DO) | Entrobacteriaceae Acinetobacter spp. Staphylococcus spp. Enterococcus spp. |
| Erythromycin (ER) | Staphylococcus spp. Enterococcus spp. |
| Furazolidone (FZ) | All organisms |
| Gatifloxacin (GF) | Entrobacteriaceae Pseudomonas aeruginosa. Acinetobacter spp . Enterococcus spp. Staphylococcus spp. Heamophilius spp. H.parainfluenzea Neisseria gonorrhoeae Streptococcus spp. |
| Gentamicin (GM) | Entrobacteriaceae Pseudomonas aeruginosa. Acinetobacter spp. Staphylococcus spp. Heamophilius spp. H.parainfluenzea |
| Imipenem\ Cilastain (IS) | Entrobacteriaceae Pseudomonas aeruginosa. Acinetobacter spp. Staphylococcus spp. Heamophilius spp. H.parainfluenzea |
| Kanamycin (KA) | Entrobacteriaceae Staphylococcus spp. |
| Levofloxacin (LV) | Entrobacteriaceae Pseudomonas aeruginosa. Acinetobacter spp. Enterococcus spp. Streptococcus spp. Stenotrophomonas maltophilia. Staphylococcus spp. Heamophilius spp. H.parainfluenzea |
| Linomycin (LN) | All organisms |
| Linezolid (LI) | Staphylococcus spp. Enterococcus spp. Streptococcus spp. |
| Lemefloxacin (LF) | Entrobacteriaceae, Pseudomonas aeruginosa Staphylococcus spp Heamophilius spp H.parainfluenzea Neisseria gonorrhoeae |
| Meropenem (MR) | Entrobacteriaceae Pseudomonas aeruginosa Acinetobacter spp. Staphylococcus spp. Burkholderia cepacia Heamophilius spp. H.parainfluenzea Neisseria meningitides |
| Minocycine (MN) | Entrobacteriaceae Acinetobacter spp. Staphylococcus spp. Enterococcus spp. Burkholderia cepacia Stenotrophomonas maltophilia. Neisseria meningitides |
| Moxifloxacin (MF) | Staphylococcus spp. Streptococcus spp. Heamophilius spp. H.parainfluenzea |
| Nalidixic acid (NA) | Entrobacteriaceae Neisseria meningitides |
| Nitrofurantoin (FU) | Entrobacteriaceae. Staphylococcus spp. Enterococcus spp. |
| Norfloxacin (NF) | Entrobacteriaceae. Pseudomonas aeruginosa. Staphylococcus spp. Enterococcus spp. |
| Ofloxacin (OF) | Entrobacteriaceae. Pseudomonas aeruginosa. Streptococcus spp. Staphylococcus spp. Heamophilius spp. H.parainfluenzea. Neisseria gonorrhoeae. |
| Pefloxacin (PF) | All organisms |
| Penicillin-G (PG) | Staphylococcus spp. Enterococcus spp. Neisseria gonorrhoeae. Streptococcus spp. |
| Piperacillin (PC) | Entrobacteriaceae. Acinetobacter spp. Pseudomonas aeruginosa |
| Piperacillin/ Tazobacum (PT) | Entrobacteriaceae. Acinetobacter spp. Pseudomonas aeruginosa. Staphylococcus spp. |
| Rifampin (RN) | Staphylococcus spp. Enterococcus spp. Heamophilius spp. H.parainfluenzea. Streptococcus pneumoniae Neisseria meningitides. |
| Roxithromycin(RO) | All organisms |
| Sparfloxacin (SP) | Staphylococcus spp. Streptococcus pneumoniae |
| Streptomycin (ST) | Entrobacteriaceae. |
| Sulfadiazine (SD | Entrobacteriaceae. Staphylococcus spp. Vibrio cholrea. |
| Teicoplanin (TP) | Staphylococcus spp. |
| Tetracycline (TE) | Entrobacteriaceae. Acinetobacter spp. Staphylococcus spp. Enterococcus spp. Vibrio cholrea. Heamophilius spp. H.parainfluenzea. Neisseria gonorrhoeae Streptococcus spp. |
| Ticarcillin/ Clavulanic acid (TC) | Entrobacteriaceae. Acinetobacter spp. Pseudomonas aeruginosa. Staphylococcus spp. |
| Tobramycin (TO) | Entrobacteriaceae. Acinetobacter spp. Pseudomonas aeruginosa. Staphylococcus spp. |
| Trimethoprim (TR) | Entrobacteriaceae. Staphylococcus spp. |
| Vancomycin (VM) | Staphylococcus spp. Enterococcus spp. Streptococcus spp. |
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